Skip to main content
. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Biochem J. 2013 Oct 1;455(1):95–106. doi: 10.1042/BJ20121755

Figure 10. Effect of SNX27 on TJ permeability is ZO-2 dependent.

Figure 10

mpkCCD cells were infected with lentivirus expressing either inducible SNX27 or control shRNA in combination with constitutive ZO-2 or control shRNA (con). ZO-2 shRNA cells were selected for GFP by flow cytometry. SNX27 shRNA expression was induced by doxycycline (1 μg/ml). (A) Protein expression was assessed post-assay by Western blotting. (B) Permeability of the indicated mpkCCD cells to 3 kDa FITC–dextran was measured. The data represent the mean of two assays from four independent experiments (one-way ANOVA, P < 0.05; Dunnett’s multiple comparison post test, *P < 0.05 and ** P < 0.01; error bars represent the S.D.).