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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Biochem J. 2013 Oct 1;455(1):95–106. doi: 10.1042/BJ20121755

Figure 3. Recruitment of ZO-2, but not ZO-1, to the early endosome by SNX27.

Figure 3

mpkCCD cells were infected with RFP–Rab5a (early endosome marker) and co-transfected with Myc–SNX27 and either YFP–ZO-2 (upper panels) or YFP–ZO-1 (lower panels) infected with RFP–Rab5a and grown to confluence. At this point, the cells were fixed/permeabilized and stained with an anti-Myc monoclonal antibody and visualized using Alexa Fluor® anti-rat 647 nm. Arrows indicate co-localized protein (scale bar = 15 μm).