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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Biochem J. 2013 Oct 1;455(1):95–106. doi: 10.1042/BJ20121755

Figure 9. Loss of SNX27 alters TJ permeability characteristics.

Figure 9

(A) SNX27 shRNA-resistant rescue strategy. (B) Resistance of SNX27r to SNX27 shRNA as revealed by Western blotting for SNX27 (upper panel). Equal loading confirmed by Western blotting for α-tubulin. (C) Flux of 3 kDa fluorescein-conjugated dextran was measured in steady-state control, SNX27 shRNA or SNX27 shRNA/SNX27r expressed mpkCCD cells. Flux of six cells per experiment were quantified (one-way ANOVA, P < 0.05; Dunnett’s multiple comparison post test *P < 0.05; error bars represent the S.D.). Con, control.