Figure 3.

SP235 immunohistochemistry of human normal and neoplastic tissues. Sections were dewaxed, microwaved in citric acid and incubated with the rabbit monoclonal anti-VPAC2 antibody (SP235) at a dilution of 1:500. Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with haematoxylin. Insets in A and D, for adsorption controls the SP235 was incubated with 10 μg/ml of the peptide used for immunisations (+ peptide). Scale bar, A=B=C=F=I=250 μm and D=E=G=H=100 μm.