Fig. 5.
Cancer cells exhibit dose-dependent sensitivity to the CPT drug photo-released from hydrogel-NP hybrid scaffolds. (a) Cell culture setup for assessing CPT drug delivery to U87 glioblastoma brain cancer cells. (b) Images of hydrogel-NP hybrid scaffolds generated in cell culture inserts. (c) Phase images of brain cancer cells after four days in varying conditions: control (untreated cells), hydrogel without NPs, hydrogel-NP without UV irradiation and hydrogel-NP with 10-min UV irradiation (365 nm, 2.5 mW/cm2). Scale bars = 50 μm. (d) Cellular viability after four days in the presence of varying NP-loaded hydrogels (n = 3), with and without UV irradiation (normalized to control untreated cells).