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. 2015 Jan 7;5:738. doi: 10.3389/fmicb.2014.00738

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Copyright © 2015 Sieger and Bramkamp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Fluorescence microscopy images of C. glutamicum DivIVA-mCherry, ΔrodA (BSC002), complemented with full length and truncated versions of RodA-GFP. RodA-GFP localizes DivIVA-like to the cell poles (first row, yellow arrows, strain BSC014). RodAΔC10-GFP forms random foci in the cell and several DivIVA foci are free of RodAΔC10-GFP, implicating reduced interaction (second row, white arrows, strain BSC015). RodAΔC80-GFP appears cytoplasmic, polar foci are not observed (third row, strain BSC016). Fluorescence images were taken at equal exposure times (250 ms for mCherry and 500 ms for GFP). Scale bars: 2 μm. (B) Growth experiment of WT, ΔrodA and complementation strains on Brain Heart Infusion agar. WT and full length complementation showed normal growth after 12 h at 30°C (Sieger et al., 2013). Complementation with RodAΔC10-GFP (strain BSC015) had a slight growth defect, reflecting the observation of reduced DivIVA interaction. ΔrodA (BSC001) as well as complementation strains RodAΔC80-GFP (BCS016), RodA1/2-GFP (BSC017), and RodA2/2-GFP (BSC018) could not develop colonies within 12 h.