Table 3.
Classification and apportioning of FRET ratios into subgroups representing the interaction situation of the fusion proteins.
| CFP/YFP ratio | Meaning | Examples |
Reference | ||
|---|---|---|---|---|---|
| Donor | Acceptor | Ratio | |||
| <0.9 | CFP fluorescence; no FRET | CFP | – | 0.55 | This study |
| ParB-CFP | – | 0.57 | This study | ||
| ParBR21A-CFP | – | 0.57 | This study | ||
| RodA-CFP | – | 0.60 | Sieger et al. (2013) | ||
| FtsW-CFP | – | 0.62 | Sieger et al. (2013) | ||
| CFP | YFP | 0.64 | This study | ||
| RodAmutC10-CFP | – | 0.67 | This study | ||
| BetP-CFP | – | 0.68 | Sieger et al. (2013) | ||
| RodAS433GS437G-CFP | – | 0.68 | This study | ||
| RodAΔC10-CFP | – | 0.73 | This study | ||
| RodAK434G-CFP | – | 0.75 | This study | ||
| RodAQ435G-CFP | – | 0.89 | This study | ||
| 0.9–1.1 | Approximation of fluorophores; no enrichment or interaction | RodA-CFP | DivIVAΔC20-YFP | 0.92 | This study |
| RodA-CFP | DivIVAK20F-YFP | 0.93 | This study | ||
| ParBR21A-CFP | DivIVA-YFP | 0.95 | This study | ||
| RodA-CFP | DivIVAK20G-YFP | 0.96 | This study | ||
| RodA-CFP | DivIVAK20I-YFP | 0.97 | This study | ||
| RodA-CFP | DivIVAΔN-YFP | 0.97 | This study | ||
| pRSETB AT1.03 | +CCCP (1 μg/ml) | 0.98 | Sieger et al. (2013) | ||
| BetP-CFP | DivIVA-YFP | 0.99 | Sieger et al. (2013) | ||
| RodAΔC80-CFP | DivIVA-YFP | 0.99 | This study | ||
| RodAΔC10-CFP | DivIVA-YFP | 1.03 | This study | ||
| RodAK434G-CFP | DivIVA-YFP | 1.04 | This study | ||
| RodAQ435G-CFP | DivIVA-YFP | 1.04 | This study | ||
| RodAS433GS437G-CFP | DivIVA-YFP | 1.06 | This study | ||
| FtsW-CFP | DivIVA-YFP | 1.09 | Sieger et al. (2013) | ||
| 1.1–1.3 | Enrichment/interaction | RodAE438G-CFP | DivIVA-YFP | 1.10 | This study |
| RodA-CFP | DivIVAK20R-YFP | 1.11 | This study | ||
| RodA-CFP | DivIVAI18D-YFP | 1.14 | This study | ||
| RodA-CFP | DivIVA-YFP | 1.15 | Sieger et al. (2013) | ||
| ParB-CFP | DivIVA-YFP | 1.16 | This study | ||
| RodAmutC10-CFP | DivIVA-YFP | 1.17 | This study | ||
| RodA-CFP | DivIVAΔM-YFP | 1.19 | This study | ||
| pRSETB AT1.03 | - CCCP | 1.25 | Sieger et al. (2013) | ||
| RodA-CFP | DivIVAI18F-YFP | 1.27 | This study | ||
| >1.3 | YFP fluorescence; no FRET | – | YFP | 2.55 | This study |
| – | DivIVA-YFP | 4.43 | This study | ||
| – | DivIVAΔN-YFP | 4.52 | This study | ||