Table 1.
Fatty acid composition of E. coli K-12 MG1655 parental and AN62 strain at atmospheric (0.1 MPa) and high (60 MPa) pressure.
| Fatty Acid | WT | AN62 | AN62 |
|---|---|---|---|
| 0.1 MPa | 0.1 MPa | 60 MPa | |
| % of total composition | |||
| 11:0 3OH | 0.04 | – | – |
| 12:0 | 6.11 | 4.60 | 3.99 |
| 12:0 3OH | 0.07 | 0.06 | 0.08 |
| 13:0 | 0.09 | 0.10 | – |
| 14:0 | 9.37 | 6.14 | 5.54 |
| 14:0 3OH | 10.76 | 8.86 | 8.13 |
| 15:0 | 0.19 | 0.13 | 0.10 |
| 16:0 | 27.72 | 34.06 | 22.73 |
| 16:0 3OH | 0.08 | 0.07 | 0.09 |
| 16:1 ω5c | 0.33 | 0.31 | 0.84 |
| 16:1 ω7c | 31.66 | 20.46 | 20.72 |
| 17:0 | 0.04 | 0.05 | 0.06 |
| 17:0 cyclo | 2.22 | 2.93 | 3.59 |
| 18:0 | 0.14 | 0.33 | 0.36 |
| 18:1 ω5c | – | 0.11 | 0.25 |
| 18:1 ω7c | 9.50 | 20.02 | 31.15 |
| 18:2 ω6,9c | 0.04 | 0.06 | – |
| 19:0 iso | 0.03 | – | – |
| 19:0 cyclo ω8c | 0.05 | 0.21 | 1.06 |
| Unknown | 1.48 | 1.48 | 1.25 |
| Sum (%) | 99.99 | 99.98 | 99.99 |
| UFA/SFA (%) | 94.97 | 90.24 | 161.32 |
Cultures were grown micro-aerobically in LB supplemented with glucose and HEPES buffer at 37°C. Cells were harvested at the early exponential growth phase. WT, parental strain; UFA, unsaturated fatty acids; SFA, saturated fatty acids.