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Bioluminescence images of NanoLuc fused with NLS (A), CoxVIII (B), calreticulin (C) or no targeting sequence (D) in U2OS cells. Images were captured using an LV200 microscope with UPlanFLN 100× Oil objective lens and ImagEM EM-CCD camera at 37°C. Exposure time, 300 ms (A, D), 500 ms (B) and 1 sec (C); Furimazine, 12.5 μM; Scale bars, 20 μm.
