Table 1.
Spectral properties of beetle (CBG99, CBR, Luc2) and deep-sea shrimp (NanoLuc) luciferases expressed in U2OS cell lines
| λ Max (nm) | ||||
|---|---|---|---|---|
| Relative Intensity | Relative Intensity/Luc2 | |||
| Cell Line | 25°C | 37°C | (37°C/25°C) | (37°C) |
| CBG99 | 548.69 (±0.95) | 549.93 (±0.36) | 3.71 (±0.24) | 1.72 (±0.11) |
| CBR | 616.62 (±0.00) | 617.44 (±0.94) | 1.51 (±0.45) | 0.83 (±0.25) |
| Luc2 | 596.47 (±0.36) | 608.80 (±0.71) | 2.02 (±0.87) | 1.00 (±0.43) |
| NanoLuc | 463.22 (±0.63) | 462.59 (±1.09) | 0.98 (±0.25) | - |
Note: Bioluminescence intensity between 25 and 37°C, and among cell lines at 37°C, was compared with the total surface area that emitted luminescence in the relevant spectrum. Mean ± SD, n = 3.