Table 1.

List of primary antibodies used in TNFR1 signaling study

NF-κB Pathway		PI3K/AKT Pathway		MAPK Pathway		Src/JAK/STAT3
Antibody	Dilution	Antibody	Dilution	Antibody	Dilution	Antibody	Dilution
NF-κB p65	1:250	AKT	1:100	P38 MAP K	1:000	c-SRC	1:000
pNF-κB p65	1:100	P-AKT (T308)	1:50	pP38 MAP	1:000	pC-SRC	1:1000
p105/p50 NF-κB	1:1000	P-AKT (Ser.)	1:25	pHSP27	1:50	Jak2	1:000
IκB-α	1:250	p-eNos	1:500	ERK1/2	1:2000	pJak2	1:1000
P-IκB-α	1:500	pC-raf	1:1000	pERK1/2	1:2000	Stat3	1:1000
IKK-α	1:250	GSK-3β	1:500	pSAPK/JNK	1:1000	pStat3	1:500
IKK-β	1:250	pGSK-3β(Ser9)	1:500	pMEK3/MEK6	1:100
A20	1:500	PDK1	1:1000	pMAPKAPK 2	1:1000
c-IAP2	1:500	pPDK1	1:1000	pSek1/MKK4	1:250	c-FLIP	1:500
pTAKI	1:100	PTEN	1:1000	pC-Jun (Ser)	1:100	P-ATF2	1:250
		pPTEN	1:1000	TRAF2	1:500	P-ELK-1	1:500
		PI3K 110	1:250			Actin (Mouse)	1:1000
		pPI3K P85	1:250			NU P98	1:1000
		pBad 136	1:000			HSP 90	1:1000

All antibodies were rabbit antibodies and were purchased from Cell Signaling Technology. For western blotting, the antibodies were diluted 1:1000 in antibody diluent (Dako). For lysate microarray, they were diluted as shown in the table in the same dilution buffer.