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. 2013 Nov 23;82(6):975–989. doi: 10.1002/prot.24472

Figure 9.

Figure 9

Binding of REX variants to human cell lines correlates with IL-23R expression levels. (a) Expression of IL-23 receptor molecules on the surface of cultured human K-562 and THP-1 cells was detected by flow cytometry using anti-human IL-23R-APC or isotype control-APC mAbs. (b) Binding of REX-TolA-AVI variants to K-562 and THP-1 cells. The cells were incubated with in vivo biotinylated REX-TolA-AVI proteins or negative ABD-TolA-AVI controls (ABDwt, ΔABD) and the cell-bound proteins were stained with streptavidin-phycoerythrin and analyzed by flow cytometry. In the n.p. control, the REX binders and ABD controls were omitted. (c) Comparison of IL-23 receptor expression on K-562 and Jurkat cell surface. The cells were stained with anti-human IL-23R-APC or isotype-APC control mAbs and analyzed by flow cytometry. (d) Comparison of REX ligand binding to K-562 and Jurkat cells. The cells were incubated with biotinylated REX variants or ABDwt control and cell-bound proteins were detected with streptavidin-phycoerythrin by flow cytometry.