Table 1. Data-collection and scattering-derived parameters.
| IgG1 | IgG2 | IgG4 | |
|---|---|---|---|
| Data-collection parameters (for all IgGs) | |||
| Instrument | EMBL X33, DORISIII (DESY, Hamburg), Pilatus 1M detector | ||
| Beam size at the detector (mm) | 2 0.6 | ||
| Wavelength () | 1.5 | ||
| q range (1) | 0.0090.500 | ||
| Exposure time (s) | 15 | ||
| Concentration range (mgml1) | 112 | ||
| Temperature (K) | 281 | ||
| Structural parameters | |||
| I(0) (cm1) (from Guinier) | 0.1220.001 | 0.1180.001 | 0.1180.001 |
| R g () (from Guinier) | 49.40.38 | 47.60.48 | 47.60.49 |
| I(0) (cm1) [from P(r)] | 0.124 | 0.120 | 0.119 |
| R g () [from P(r)] | 51.0 | 49.4 | 49.0 |
| D max () | 162 | 162 | 158 |
| Porod volume estimate (3) | 250720 | 239520 | 248080 |
| Dry volume (3)† | 176829 | 176380 | 176434 |
| Molecular-mass determination | |||
| Correlation length () | 0.12 | 0.13 | 0.13 |
| Volume of correlation (2) | 939 | 908 | 910 |
| Q R (3) | 17857 | 17338 | 17397 |
| Molecular mass M r (Dalton) (from Q R) | 145000 | 141000 | 141000 |
| Corresponding particle density (gcm3) | 0.96 | 0.97 | 0.95 |
| Monomeric M r † (Dalton) | 145000 | 145000 | 145000 |
| Software employed (for all IgGs) | |||
| Primary data reduction | PRIMUS | ||
| Data processing | PRIMUS, SCTTER | ||
| Homology modelling | SWISS-MODEL | ||
| Rigid-body modelling | CORAL | ||
| Ensemble modelling | EOM | ||
| Shape clustering | DAMCLUST | ||
| Computation of model intensities | CRYSOL | ||
| Three-dimensional graphics representations | PyMOL | ||
†
Calculated from the sequence.