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Membrane extractability of CtsP-FLAG and His-CtsX. Membranes were incubated with the indicated reagents for 30 min at 4°C and sedimented by ultracentrifugation. Separated fractions were examined by 12% or 15% SDS-PAGE. Fusion proteins were detected by immunoblotting with anti-FLAG M2 antibody for CtsP-FLAG and with an anti-6×His antibody for His-CtsX. S, soluble fraction after washes; M, membrane fraction.
