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. 2015 Jan 7;197(3):615–625. doi: 10.1128/JB.02080-14

FIG 6.

FIG 6

MS/MS fragmentation and confirmation of the amino acid sequence of the M. leprae rSodC glycopeptides. (A) The MS/MS fragmentation of 1,068.5024 m/z (M + 3H)3+ corresponding to the 51TGSPAPSGMLGAEAESMGPPNIITRL76 glycopeptide (Fig. 5A) obtained with a collision energy of 42.0 eV provided b and y series ions that confirmed the amino acid sequence of the peptide. (B) MS/MS of the 1,121.4571 m/z (M + 2H)2+ corresponding to the 51TGSPAPSGMLGAEAESM67 glycopeptide (Fig. 5B) obtained with a collision energy of 44.0 eV provided b and y series ions that confirmed the amino acid sequence of the peptide. MS/MS ion data were deconvoluted using the Mass Hunter Qualitative Analysis software, version B.04.00, and the amino acid caliper was used to identify the probable amino acid fragmentation differences between the dominant ions in the spectra.