TABLE 2.

Primer sequences used in this study

Primer use and gene or site targeteda	Primer	Primer sequence
Construction of lux reporters
ldtD	pJW5-ldtD(R)	5′-AAAGTAATTCTTTGCGTGCGGGGCTTT-3′
ldtD	pJW5-ldtD(F)	5′-AAACTCGAGTGGTACAAAGCTGGGAAGAT-3′
ygaU	pJW5-ygaU(R)	5′-AAAGAAATTCTACCGGTTTGTTCAGATGC-3′
ygaU	pJW5-ygaU(F)	5′-AAACTCGAGGGAACCGCTAAGCATGCACA-3′
slt	pJW5-slt70(R)	5′-AAAGAATTCTCAATGGACTCACGCACGGT-3′
slt	pJW5-slt70(F)	5′-AAAGGATCCTGCTCATCCAGTGAGTCGGC-3′
Verification of the MCS in pJW15
MCS-pJW15	MCS-pJW15(R)	5′-CACCAAAATTAATGGATTGCAC-3′
MCS-pJW15	MCS-pJW15(F)	5′-GCTTCCCAACCTTACCAGAG-3′
Amplification of gene promoters for EMSA
degP	PdegP-F	5′-CGCTTATTCCACAAACTCTCG-3′
degP	PdegP-R	5′-CGGCTGAGACTTCTTCAGCAACGA-3′
rpoD	PrpoD-F	5′-CGAAGAACGCCTGGAGC-3′
rpoD	PrpoD-R	5′-TCACCTGAATGCCCATGTCG-3′
ygaU	PygaU-F	5′-AAATGCTGTGATGTCGCAGAGG-3′
ygaU	PygaU-R	5′-GCGTCCCAGAGTTTTTCTCC-3′
ldtD	PldtD-F	5′-AAATGGTACAAAGCTGGGAGAT-3′
ldtD	PldtD-R	5′-AAATTTGCGTGCGGGCTTTTTCT-3′
slt	Pslt70-F	5′-AAATCCTGCGGCAGATAACCAAT-3′
slt	Pslt70-R	5′-AAATCGGTGATCTGGCGGTATTC-3′
KEIO or deletion verification
ldtD	KldtD-F	5′-AAAGTGGCACATTACGGCG-3′
ldtD	KldtD-R	5′-AAAGAGAGTGTTGCAAACGCAGG-3′
ygaU	KygaU-F	5′-AAATCGGTAAAGGGTTCGGTTGG-3′
ygaU	KygaU-R	5′-AAATAAGCTTGAGGCCTGTGACG-3′
slt	Kslt70-F	5′-AAATCCTGCGGCAGATAACCAAT-3′
slt	Kslt70-R	5′-AAATCGGTGATCTGGCGGTATTC-3′
cpxA* (cpxA24)	cpxA*-F	5′-ATGACCGAGCTTCTGGATAGC-3′
cpxA* (cpxA24)	cpxA*-R	5′-CTTCGCCATCACGGCACGGGAG-3′

^aMCS, multiple-cloning site.