TABLE 2.
Primer list
| Namea | Sequenceb | Enzyme |
|---|---|---|
| PrapP-F | AAGTGAATTCTTCATCCGGAGACTATTTAT | EcoRI |
| PrapP-R | GTGGGGATCCTCAAATACCTCCTTTTCTTT | BamHI |
| PphrP-F | AAGTGAATTCCGCTTTAGAAACAGCTGAAA | EcoRI |
| PphrP-R | GTGGGGATCCATGAAGCGCTAGATACTCCA | BamHI |
| PrapA-F | CGACCGAATTCCAAAACTTACAGAAGGCTT | EcoRI |
| PrapA-R | GCAGAGCTAGCTGAATTACCCGAGATATGTC | NheI |
| Peps-F | GTGCGGAATTCGTCGTTATTTCGTTCATTAT | EcoRI |
| Peps-R | AGTTGGCTAGCTCATGTATTCATAGCCTTCA | NheI |
| Psrf-F | ATGGGGAATTCCGTTGTAAGACGCTC | EcoRI |
| Psrf-R | AGGTGGCTAGCTTTATAAGCAGTGAACAT | NheI |
| rapP-phrP-F | AGGAGGATATCTTCATCCGGAGACTATTTATGAACAA | EcoRV |
| rapP-phrP-R | CTCCTGCATGCTTAGGTGGTAGCACCATTCTTGCA | SphI |
| rapP-R | ATGAAGCATGCTTACATTTTTTCATTTAAATG | SphI |
| hsRapP-F | ATCCAGCTAGCAAAGAAAAGGAGGTATTTGATTG | NheI |
| hsRapP-R | ATCCGGCATGCCAAGAGCGCTAAACAAATTG | SphI |
| hsPhrP-F | ATCCAGCTAGCAAATTCAAAGGGGGAAACATTTAAATG | NheI |
| hsPhrP-R | ATCCGGCATGCTTAAGTTGCTGCTCTATCTG | SphI |
| rapP-phrP-NtoT-F | [phos]AGCTCATTTTAACGTGGGATTA | |
| rapP-phrP-NtoT-R | [phos]GAACCAATTAAATGTTGCTCCC | |
| comA-P1 | AAGTTGGACCGGACTGGAAT | |
| comA-P2 | TTTTCTAATGTCACTAACCTGCCAAACTGTTCGCTCGGTTCAG | |
| comA-P3 | AGTAATCCGCCCGACGGTATAGCGGTCCATTGAATACAGC | |
| comA-P4 | GGTGAGCCGGTGATGTTTAC |
a
See Materials and Methods for descriptions. Primer rapP-phrP-NtoT-F contains the mutated base necessary for creating the T236N substitution by performing a C-to-A mutation. This base is shown in boldface in the sequence. F, forward; R, reverse.
b
The restriction enzyme recognition sequences are underlined. [phos] indicates the 5′ phosphorylated primer.