FIG 2.
Recombinant in vivo M. tuberculosis reporter assay using M. tuberculosis σE and its specific promoter, sigBpr. (A) The strategy for this experiment was the same as that shown in Fig. 1A, except sigA was replaced by sigE in the dual-plasmid system and sinP3 was preplaced by sigBpr in pFPVmCherry. (B) Results for recombinant M. tuberculosis reporter assays: The bars represent mCherry fluorescence of E. coli cells containing pFPVmCherry-sigBpr plasmid in the presence of the following: no M. tuberculosis RNAP (control); M. tuberculosis RNAP core (pAcYc Duet rpoA plus pCOLA Duet rpoB-rpoC); M. tuberculosis RNAP-sigE holo (as in panel A); σE (pAcYc Duet-sigE). The data represent means of three replicates, and error bars represent standard deviations.