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TLC showing the accumulation of two new glycolipids in E. coli expressing Agt from Mesorhizobium. The two glycolipids were further analyzed by subsequent NMR spectroscopy and identified as MGlcD and GlcAD. No glycolipids were observed in the empty vector control. Glycolipids were stained with α-naphthol–H2SO4 at 137°C.
