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Characterization of OlsD as the responsible enzyme for OL-OH formation in Mesorhizobium. (A) Two-dimensional TLC of a lipid extract from Mesorhizobium ΔolsD grown under phosphate deprivation lacking OL-OH. (B) Separated lipid extract from Agrobacterium as a host for heterologous expression of OlsD grown under phosphate-replete conditions with accumulation of two novel hydroxylated ornithine lipids, OL-OH and OL-(OH)2.
