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Linear representation of ToxT domain orientation and mutagenesis sites. Amino acids located in the unstructured region (UR) of ToxT between amino acids 100 and 110 were mutated to alanine for initial analysis of the region. The N-terminal domain (NTD) is located between amino acids 1 and 160, the linker (L) between amino acids 161 and 169, the C-terminal domain (CTD) between amino acids 170 and 276, and helix-turn-helix domains 1 and 2 (HTH1 and HTH2) within the CTD.
