FIG 11.

Linoleic acid inhibits ToxT proteolysis. (A) WT ToxT was produced from pBAD-toxT (29) under virulence-repressing conditions (LB, pH 8.5, 37°C, shaking) for 4 h, leading to production of the proteolytic intermediate (gray arrow). Full-length WT ToxT (black arrow) is produced under each condition. Control DMSO does not affect proteolysis (lane 3). The addition of increasing concentrations of linoleic acid blocked the formation of the ToxT degradation product (lanes 5 and 6). Palmitic acid, virstatin, and bicarbonate did not affect ToxT proteolysis (lanes 7 to 10). Blots were probed with mouse monoclonal anti-His tag antibody.