FIG 13.

Linoleic acid inhibits proteolysis of effector-insensitive ToxT mutants. (A) Degradation of WT ToxT and R105A, N106A, and N106F mutants with DMSO (−) and with 320 μM linoleic acid (+) is shown. Linoleic acid inhibits proteolysis with each of the mutations (lanes 4, 6, 8, and 10). The R105A mutant still showed a small band of degraded ToxT in the presence of linoleic acid (lane 6). Samples were normalized by OD₆₀₀. Experiments were performed a minimum of three times, and a representative blot is shown. (B) Quantification of band intensities from the blot shown in panel A was performed by densitometry using ImageJ software. Graph represents percentages of ToxT intermediate compared to total ToxT protein.