FIG 8.

TEM analysis of biofilm mutants. We used TEM to visualize wrinkled and smooth colonies formed by applying the following pKG11-containing strains to LBS medium containing Tc: wild type (ES114) (A), bmpABC mutant (KV6897) (B), and sypL mutant (KV5069) (C). (D) We also collected samples from the interface of touching cultures of the pKG11-containing bmpABC and sypL mutant strains. Arrows indicate the thread-like material (likely a polymer or polysaccharide), arrowheads indicate outer membrane vesicles, and a star indicates the “swollen cell” phenotype (present only in the sypL mutant strain). Bars, 100 nm.