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. 2013 Nov 26;37(5):1144–1158. doi: 10.1111/pce.12223

Figure 2.

Figure 2

Generation of Ubi::OsBURP16 transgenic rice. (a) Diagram of the Ubi::OsBURP16 construct that was used for the generation of transgenic plants. (b) Southern blot analysis of overexpression transgenic rice lines. ZH10, wild type Zhonghua10; OE3, OE4, OE6 are the overexpression transgenic lines 3, 4 and 6. Rice genomic DNA was digested by EcoRI in the left four lanes and by HindIII in the right four lanes. (c) The expression levels of OsBURP16 in OsBURP16-overexpressing lines (OE3, OE4, OE6) were determined by qRT-PCR. The expression level in ZH10 was normalized as 1. The number in each column indicates the expression levels of OsBURP16 in each transgenic line. Data represent means ± SD of three biological replicates. (d–g) Phenotypes of 2-week-old seedlings (d), plants at heading stage (e), spikelets (f) and seeds (g) of ZH10 and OsBURP16 line OE3 and OE4. Scale bar = 2 cm (d), 10 cm (e), 5 cm (f) and 1 cm (g).