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. 2013 Nov 26;37(5):1144–1158. doi: 10.1111/pce.12223

Figure 4.

Figure 4

Performance of Ubi::OsBURP16 transgenic rice under cold stress. (a) Seedlings of OsBURP16-overexpressing lines (OE) and wild-type (ZH10) at trefoil stage were treated at 4 °C for 8 h. The leaves of lines OE3 and OE4 started rolling, while the leaves of ZH10 remained normal. The boxed parts in the top panel are enlarged as a detail (lower panel). Scale bar = 5 cm. (b) Two-week-old seedlings were treated at 4 °C for 72 h, and then allowed to recover for 7 d. Scale bars = 5 cm. (c) Survival rates of the cold-treated plants after 7 d of recovery. Data represent means ± SD (n = 16 for each replicate) of three independent experiments. One asterisk and two asterisks indicate significant differences at P ≤ 0.05 and P ≤ 0.01 compared with ZH10 by Student's t-test, respectively. (d) Relative ion leakage in rice leaves after 4 °C treatment for 60 h and 108 h. Data represent means ± SD of three biological replicates. One asterisk indicates significant difference at P ≤ 0.05 compared with corresponding ZH10 by Student's t-test.