Figure 3.

Correlation between the array seroreactivity and previously published studies. (A) Venn diagram of the leptospiral proteins identified by mass spectrometry when the bacteria were grown in vitro (red); proteins for which the corresponding mRNA was up-regulated when the bacteria were cultivated in dialysis membrane chambers in vivo (green); and antibody reactivity in leptospirosis patients detected by protein microarray (blue). (B) Scatter plots with the antibody reactivity and number of protein copies/cell detected by mass spectrometry. Array average signal intensity for IgM or IgG probing is plotted on the X axis; the number of protein copies per cell detected in vitro by mass spectrometry is shown on the Y axis.