Figure 4.
(a) PCoA illustrating the differences in the endosymbiotic methanogens associated with different rumen protozoal fractions obtained from holotrich-monofaunated (H) and totally faunated sheep (F). Big circles indicate the 90% confidential interval. (b) Dendrogram depicting the effect of total protozoa fractionation on their endosymbiotic methanogen populations. Protozoa fractions (F80, F60, F45, F35, F20, F5 and F < 5) were generated by a sequential filtration of rumen fluids from different sheep (A, B, C and D) through nylon meshes with a pore size of 80, 60, 45, 35, 20 and 5 μm, respectively.