FIG 2.

Effect of individual and combined resveratrol, quercetin, and catechin (RQC) on Akt, AMPK, and p70S6K phosphorylation in MDA-MB-231 cells. Confluent MDA-MB-231 cells were serum-starved for 24 h, treated with Veh or 5 μM Res, Quer, Cat, or combined Res, Quer, and Cat at 5 μM each (RQC) for 15 min, lysed immediately, and western blotted for active and total proteins: phospho-Akt^Ser473 and Akt, phospho-AMPK^Thr172 and AMPK, or phospho-p70S6K^Thr389 and p70S6K. A: Upper panel, representative western blots (from 4 separate experiments); lower panel, Akt activity (phospho-Akt/Akt) as quantified from Image J analysis of integrated density of positive bands. B: Upper panel, representative western blots (from 4 separate experiments); lower panel, AMPK activity (phospho-AMPK/AMPK) as quantified from Image J analysis of integrated density of positive bands. C: Upper panel, representative western blots (from 4 separate experiments); lower panel, p70S6K activity (phospho-p70S6K/p70S6K) as quantified from Image J analysis of integrated density of positive bands. An asterisk indicates statistical significance (p≤0.05) when compared to vehicle.