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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Cancer Res. 2014 Mar 3;74(8):2316–2327. doi: 10.1158/0008-5472.CAN-13-2433

Figure 7.

Figure 7

HO-3867 inhibits migration and invasion, and is cytotoxic to primary ovarian cancer cells. A, STAT3 expression in primary ovarian cell populations. B, three different primary ovarian cancer cell lines were treated with 5 or 10 μmol/L of HO-3867 for 24 hours and subjected to clonogenic assay. Quantification of clonogenic survival in cells showing decreased, dose-dependent survival in all cell lines when compared with control (n = 3, reported% ± SEM, P < 0.0001 all groups vs. control). C, cell migration/wound healing assay representative images using primary ovarian cell lines evaluated before and after 24 hours exposure to 10 μmol/L of HO-3867 with corresponding controls. D, the residual gap between the migrating cells from the opposing edges is expressed as a percentage of the initial, scraped area. HO-3867 significantly inhibits the reduction in gap size caused by cell migration (n =5, control, treated, mean ± SD; all *, P < 0.0001). E, representative images of the invasion assay results using a transwell Boyden chamber with primary ovarian cancer cell lines and corresponding controls. F, quantification of invasion assay. HO-3867 inhibits invasion versus control in all cell lines (n = 3, mean ± SD; *, P < 0.0001).