Figure 3. Bosutinib and dasatinib induce a gene expression programme directed by the transcription factor CREB.
Bone-marrow-derived macrophages were treated with vehicle control, 3 μM bosutinib or 0.3 μM dasatinib for 1 h and then stimulated with LPS for the indicated times. Expression of different CREB-dependent genes, (A) IL-10, (B) nurr77 and (C) c-FOS, were measured by qPCR. mRNA levels were normalized to 1 in unstimulated cells (mean±S.E.M., n=4).