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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Fungal Genet Biol. 2014 Jul 7;70:12–23. doi: 10.1016/j.fgb.2014.06.003

Figure 2.

Figure 2

High temperature growth and auxotrophic analysis. Wild type (KN99α), ura4::NeoR (FGC003), reconstituted strain ura4Δ+URA4 (FGC003R1), ura5 mutant and the original insertional mutant G368 were incubated on the following media at 30°C and 37°C for 48 hours: YPD; YNB supplemented with 2% glucose, 5 g/L ammonium sulfate, and amino acids (YNB), with or without addition of uracil (20μM) or uridine (20μM); and 5-FOA (5-fluoroorotic acid) medium (10 mg/mL). From left to right the amount of cells inoculated in each spot is: 1 × 104, 1 × 103, 1 × 102, 1 × 101 and 1 cell.