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. 2015 Jan 7;10(1):e0115864. doi: 10.1371/journal.pone.0115864

Figure 1. Expression of defense-related genes after treatment with HM.

Figure 1

The 28- to 30-day-old Arabidopsis thaliana Col-0 plants were sprayed (A, C) or soil drenched (B) with 250 and/or 1250 ppm. Aboveground tissues were collected 0–7 (A, C) or 0–3 (B) days after treatment. Total RNA was extracted, and first-strand cDNA was synthesized for expression analysis. Expression levels of the PR-1, PDF1.2, PR-2 (glucanase; BGL2), basic chitinase PR-3, and chitinase (At2g43620; CHI620, At2g43570; CHI570) genes were monitored by qRT-PCR. The expression level of each gene was normalized against the expression level of CBP20, which is constitutively expressed. Relative expression ratios are shown as fold induction relative to the expression level at 0 h. Each experiment was repeated at least three times. Bars indicate the standard error (SE). The nucleotide sequences of the gene-specific primers for each gene are listed in Table 1.