Figure 6. Effect of HM application on Colletotrichum higginsianum in Arabidopsis plants.
The 28- to 30-day-old A. thaliana Col-0 plants were sprayed (A) or soil drenched (B) with water (control) or the indicated concentrations of HM at 2 days prior to spray inoculation with a spore suspension (5 × 105 spores mL−1) of C. higginsianum. Pathogen growth was determined 5 days after inoculation by assessing C. higginsianum actin mRNA by qRT-PCR. Bars indicate the standard error (SE). The asterisk indicates a significant difference compared with the control (Dunnett’s method [35], P < 0.05). The experiment was repeated at least twice with similar results.