Figure 7. Induction of resistance to C. higginsianum by HM application in Arabidopsis defense signaling-defective mutants.
The 28- to 30-day-old A. thaliana mutants were sprayed with water (control) or 1250 ppm HM at 2 days prior to spray inoculation with a spore suspension (5 × 105 spores mL−1) of C. higginsianum. Pathogen growth was determined 5 days after inoculation by assessing C. higginsianum actin mRNA by qRT-PCR. Bars indicate the standard error (SE). The asterisk indicates a significant difference compared with the control (Dunnett’s method [35], P < 0.05). The experiment was repeated at least twice with similar results.