Figure 4.

a. Maximum force to failure, F_max, versus days PF, with 95% CIs labeled. No significant increase in F_max was found over time in intact femurs of both Pten-CKO and WT (slope=1.1, 95% CI: -3.5, 5.7, p=0.66). F_max increased throughout healing in callus Pten-CKO and WT femurs with a slope 10.8 units higher than intact femurs (95% CI: 4.3, 17.4, p=0.004). By day 28 PF, F_max in callus Pten-CKO and WT femurs were 23% (p=0.006) and 32% less than their corresponding intact bones, respectively. At 28 days PF, F_max of intact and callus Pten-CKO femurs were significantly larger than their WT counterparts (p=0.001, p=0.017).

b. Maximum bending stress, σ_max, versus days PF, with 95% CIs labeled. No significant increase in σ_max was found over time in intact femurs of both Pten-CKO and WT (slope=4.8, 95% CI: -1.9, 11.5, p=0.17). σ_max increased over time in callus femurs of both the Pten-CKO and WT femurs with a slope 10.7 units higher than intact femurs (95% CI: 1.3, 20.1, p=0.036). For all days PF, σ_max of the callus femurs of both the Pten-CKO and WT were significantly less than intact femurs (RM-ANOVA p<0.001). By day 28 PF, σ_max in callus Pten-CKO and WT femurs were 63% and 62% less than their corresponding intact femurs, respectively.

c. Minimum section modulus, z_min, versus days PF, with 95% CIs labeled. z_min did not change with days PF for any of the groups (p=0.56). z_min of intact and callus Pten-CKO femurs were nearly twice as large as their respective WT counterparts for all days PF. Further, callus femurs of both Pten-CKO and WT were more than twice as large as their respective contralateral intact femurs.