Figure 5.

Exogenous Pcsk9 prevents SRT3025-induced increase in Ldlr expression and activity in AML12 hepatocytes. (A) Western blot and corresponding quantifications of AML12 cells treated with vehicle (Veh, DMSO) or 10 μM SRT3025 and incubated with or without Pcsk9 active protein (3 ng ml⁻¹) for 1 h. (B) BODIPY-labelled LDL uptake in AML12 cells incubated for 24 h with 10 μM SRT3025 or Veh and incubated with or without Pcsk9 active protein (3 ng ml⁻¹) for 1 h. Fluorescence intensity and western blot quantifications are given as percentage of Veh control. BODIPY, 4,4-difluoro-3a,4a-diaza-s-indacene; AU, arbitrary units; Pcsk9, proprotein convertase subtilisin/kexin type 9.