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. 2014 Mar 6;36(1):51–59. doi: 10.1093/eurheartj/ehu095

Figure 6.

Figure 6

Sirt1 knockdown reduces and Sirt1 overexpression enhances SRT3025-induced increase in Ldlr expression in AML12 hepatocytes. (A) Western blots of Ldlr, Sirt1, and β-actin with corresponding quantifications of AML12 cell lysates following transfection with Sirt1 siRNA or scrambled siRNA for 24 h and incubated with 10 μM SRT3025 or vehicle (Veh, DMSO) for additional 24 h. (B) Western blots of Ldlr, Sirt1, and β-actin with corresponding quantifications of AML12 cell lysates following Sirt1 overexpression plasmid or scramble control for 24 h and incubated with 10 μM SRT3025 or Veh for additional 24 h. Western blot quantifications are given as a percentage of Veh control. Scr, scramble plasmid; Sirt1 OE, Sirt1 overexpression plasmid.