Table 3. Effect of R Substitution on the Potency of Thiophene Inhibitors^a.

^aOne asterisk indicates the values represent the mean of three independent measurements in TEVC assays. We typically see no more than 5% variation in the percent inhibition on a given day, or 10% error for measurements made on different days with different batches of oocytes. All compounds were initially tested at 100 μM. The compounds that showed >80% inhibition at 100 μM were further tested at 30 μM. The data are presented as % inhibition at 100 μM/% inhibition at 30 μM. N.T. = not tested. Two asterisks indicate CC₅₀ was measured using confluent monolayer Madin–Darby canine kidney (MDCK) epithelial cells. Cell viability was quantified after 72 h by MTT.⁶¹