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. 2014 Aug 27;66(2):603–611. doi: 10.1093/jxb/eru345

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Induced quiescence enhances over time storage of the EPN Heterorhabditis bacteriophora. Quality traits were better conserved over time when EPNs were stored in a solution with root-cap exudates from green pea cv. Lancet (grey circle) as compared to EPNs stored in water (white circle). (A) EPN infectiousness remained significantly higher in root-cap exudates as compared to water during most of the experiment. (B) When stored in root-cap exudates, EPNs stayed mobile for longer compared to the EPNs in water over most of the duration of the experiment. (C) Because EPNs stored in root-cap exudate were quiescent, their lipid reserves were maintained at a significantly higher level over time compared to lipid reserves measured in EPNs stored in water. (D–F) After being stained with Oil Red O, lipids appear in red inside the nematode body. A visual assessment allows an evaluation of the lipid content according to the 1–8 lipid index (Patel et al., 1997). According to this index, (D) fully stained EPNs were given an 8, (E) intermediately stained EPNs received a 4, and (F) individuals without staining were recorded as 1. Level of significance between treatments on one day are indicated as not significant (ns), P < 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***). Statistical differences within treatments over time are noted with letters. Bars indicate SEM.