Table 1.
Amino acid | CO2 | Temperature | Treatment | Temperature×CO2 | Treatment× Temperature | |||||
---|---|---|---|---|---|---|---|---|---|---|
F 1,6 | P | F 1,6 | P | F 5,264 | P | F 1,6 | P | F 5,264 | P | |
Alanine (Ala)1 | 28.26 | 0.002 | – | – | 3.48 | 0.005 | – | – | – | – |
Argininea (Arg)2 | – | – | 14.41 | 0.009 | 2.41 | 0.037 | – | – | – | – |
Aspartic acid (Asp)2 | – | – | 22.9 | 0.003 | 3.62 | 0.004 | – | – | – | – |
Cysteine (Cys)4 | – | – | – | – | – | – | – | – | – | – |
Glutamic acid (Glu)3 | 10.33 | 0.02 | 17.23 | 0.006 | 5.78 | <0.001 | – | – | – | – |
Glycine (Gly)1 | – | – | – | – | – | – | 8.99 | 0.024 | – | – |
Histidinea (His)2 | – | – | 11.66 | 0.014 | 4.79 | <0.001 | – | – | – | – |
Isoleucinea (Ile)1 | – | – | – | – | 2.77 | 0.019 | – | – | – | – |
Leucinea (Leu)1 | 9.22 | 0.023 | – | – | – | – | – | – | – | – |
Lysinea (Lys)1,2,3,5 | 23.91 | 0.003 | – | – | – | – | – | – | 10.88 | <0.001 |
Methioninea (Met)4 | – | – | – | – | 3.83 | 0.002 | 15.49 | 0.008 | – | – |
Phenylalaninea (Phe)1 | 10.64 | 0.017 | – | – | – | – | – | – | 3.74 | 0.003 |
Proline (Pro)1 | 7.43 | 0.034 | – | – | 2.36 | 0.04 | – | – | – | – |
Serine (Ser)1 | 9.25 | 0.023 | – | – | 2.31 | 0.045 | – | – | – | – |
Threoninea (Thr)1 | – | – | – | – | – | – | – | – | – | – |
Tyrosine (Tyr)5 | – | – | – | – | – | – | – | – | 6.99 | <0.001 |
Valinea (Val)1 | – | – | – | – | – | – | – | – | – | – |
Treatments that were not significant (–) and interactive effects that are not displayed were absent from the final models. All dependent variables were log transformed to standardize residuals. Superscript numbers indicate groupings used for PERMANOVA based on correlations (>0.5).
a Essential amino acids as defined by Morris (1991).