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. 2014 Nov 16;66(2):541–548. doi: 10.1093/jxb/eru450

Fig. 1.

Fig. 1.

Maximal down-regulation of target genes targeted by plant-mediated RNAi occurs within 8 d post-first day exposure to transgenic Arabidopsis thaliana plants producing dsRNAs. GPA were reared on dsRNA-expressing plants over a 16 d time series. Aphids were harvested at 0, 4, 8, 12, and 16 d to test for target gene down-regulation by qRT-PCR. Bars represent average expression of the corresponding target gene at each time point for aphids reared on dsRack1 (white), dsMpPIntO2 (grey), or dsMpC002 (black) compared with aphids reared on dsGFP. Data represent mean expression levels ±SD for each target gene at each time point for three biological replicates. Relative target gene expression values were normalized to the expression level of dsGFP aphids set at 1. Asterisk indicates significant difference compared with the dsGFP control (Student’s t-test, n=3, P<0.05), and P-values (to three decimal places) corresponding to individual data points are displayed above each bar.