Skip to main content
. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Biochemistry. 2013 Aug 16;52(34):5952–5965. doi: 10.1021/bi400696j

Figure 1.

Figure 1

Global fitting to AR progress curves, and UV-vis detection of the quinonoid intermediate. (A) Global nonlinear regression fitting of the reversible Michaelis-Menten mechanism to progress curves at pH 8.9 and 25 °C. Experimental data are in black and fitted curves are in red. The Michaelis-Menten parameters in Table 1 were derived from this fit. (B) Difference spectrum of AR at pH 8.9 and 25 °C: 3.5 mM AR in the presence of saturating D, L-alanine, minus AR without alanine. A positive peak near 500 nm is indicative of the quinonoid intermediate.