Table 1.
Annotations of “Metabolites of Interest” Detected in Medicago Root Nodules
| Metabolite | Measured m/z |
Theoretical m/z |
Δ ppm |
|---|---|---|---|
| Aminobutryic acid | 104.0708 | 104.0706 | 1.9 |
| Methyl-piperidin-iumone [M+] | 114.0915 | 114.0913 | 1.4 |
| Proline | 116.0706 | 116.0706 | 0.0 |
| Aminopentene-diol* | 118.0863 | 118.0863 | 0.0 |
| Isoleucinol | 118.1228 | 118.1226 | 1.3 |
| Asparagine* | 133.0607 | 133.0608 | 0.8 |
| Glutamic Acid | 148.0603 | 148.0604 | 0.9 |
| Asparagine [M+Na]* | 155.0425 | 155.0427 | 1.3 |
| 3-thiophen-1-yl propanoic acid | 158.0397 | 158.0396 | 0.7 |
| Ethyl-aminocyclopentane carboxylic acid | 158.1173 | 158.1176 | 1.6 |
| Phenylalanine | 166.0859 | 166.0863 | 2.1 |
| Asparagine [M+K]* | 171.0165 | 171.0167 | 1.2 |
| Arginine | 175.1187 | 175.1190 | 1.4 |
| Tyrosine | 182.0810 | 182.0812 | 1.0 |
| Methyl-alpha-galactopyranoside | 195.0862 | 195.0863 | 0.6 |
| Ethyl-aminocyclopentane carboxylic acid [M+K] | 196.0731 | 196.0734 | 1.7 |
| Proclavaminic acid* | 203.1024 | 203.1026 | 1.0 |
| Methyl-alpha-galactopyranoside [M+Na] * | 217.0678 | 217.0682 | 1.8 |
| Proclavaminic acid [M+Na] | 225.0840 | 225.0846 | 2.4 |
| Proclavaminic acid [M+K] | 241.0577 | 241.0585 | 3.3 |
| Dihydroxybenzoic acid succinimido ester | 252.0500 | 252.0503 | 1.2 |
| 5-amino-2-(aminomethyl)-6-butoxyoxane-3,4-diol [M+Na] | 257.1462 | 257.1472 | 3.9 |
| 9H-fluoren-9-yl-di(propan-2-yl)phosphane | 283.1618 | 283.1610 | 2.9 |
| N-(4-guanidinobutyl)-3-methyldecanamide | 299.2802 | 299.2805 | 1.1 |
| SAM (S-adenosyl-L-methionine) | 399.1441 | 399.1445 | 1.1 |
| 2-hydroxy-5-[[2-phenyl-2-[4-(phenylcarbamoylamino) phenyl]sulfanylacetyl]amino]benzoic acid | 514.1423 | 514.1431 | 1.5 |
| 2-[hydroxy-[(2R)-3-hydroxy-2-[(9E,12E)-octadeca-9,12-dienoyl] oxypropoxy]phosphoryl] oxyethyl-trimethylazanium [M+] | 520.3395 | 520.3398 | 0.5 |
| Oleoyl lysophosphatidylcholine | 522.3552 | 522.3554 | 0.5 |
| 18-[(4Z)-4-[(2-hydroxy-5 -nitrophenyl)hydrazinylidene] -3 -oxocyclohexa-1,5-dien-1-yl]octadecanoic acid [M+] | 541.3125 | 541.3146 | 3.9 |
| 3-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxypropanoic acid | 580.0223 | 580.0242 | 3.1 |
| [[(2R,3S,5R)-5-[4-amino-5-(4-aminobutyldisulfanyl)-2-oxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate [M+] | 602.0052 | 602.0067 | 2.4 |
| (Z)-4-oxo-2-[(Z)-1-oxooctadec-9-enyl]-12-henicosenoic acid | 603.5340 | 603.5347 | 1.1 |
| 1,3-dilinolenin | 613.4814 | 613.4827 | 2.0 |
| Heme | 617.1844 | 617.1846 | 0.4 |
Ions are [M+H] unless otherwise specified.
*
Found in both MALDI and ESI