(A) Immunofluorescence staining for coexpression of KLF5 (red) and Ki67 (green). DAPI (blue) was utilized as a nuclear stain.(B) Immunofluorescence staining of the crypt with DAPI (B) and without DAPI (C) show regions of Klf5 and Ki67 expression overlap. Colocalization between these two proteins is seen in yellow. (D-H) Regional histological analysis of tamoxifen-induced recombination in Klf5loxP/loxP; VilWT control mice (D) and Klf5loxP/loxP; VilCreERT2 experimental mice 3 (E), 5 (F), 14 (G), and 28 days (H). (I) Comparison of Klf5 mRNA expression analysis by RT-qPCR from jejunal segments of the small intestine. Student's t-test was performed to determine p-values; expression was normalized to Actb. (Mean ± SEM: P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n = 3-6).