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. Author manuscript; available in PMC: 2016 Jan 1.

Published in final edited form as: Dig Dis Sci. 2014 Jul 29;60(1):86–100. doi: 10.1007/s10620-014-3307-z

Histological comparison of Klf5^loxP/loxP; Vil^WT (WT) and Klf5^loxP/loxP; Vil^CreERT2 (Klf5-mutant) intestinal tissues for cellular differentiation utilizing immunofluorescent staining for chromagranin A (green; enteroendocrine cells) (A-C),mucin2 (green; goblet cells) (D-F), lysozyme (green; Paneth cells) (G-I), and dipeptidyl peptidase-4 (green; enterocytes) (J-L). Nuclear stain is DAPI (blue). (M) Comparison of ChgA, Muc2, Gata4, and Fabp1 mRNA expression analysis by RT-qPCR from jejunal segments of the small intestine. Student's t-test was performed to determine p-values; expression was normalized to Gapdh. (Mean ± SEM: P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n = 3-6).

Histological comparison of Klf5^loxP/loxP; Vil^WT (WT) and Klf5^loxP/loxP; Vil^CreERT2 (Klf5-mutant) intestinal tissues for cellular differentiation utilizing immunofluorescent staining for chromagranin A (green; enteroendocrine cells) (A-C),mucin2 (green; goblet cells) (D-F), lysozyme (green; Paneth cells) (G-I), and dipeptidyl peptidase-4 (green; enterocytes) (J-L). Nuclear stain is DAPI (blue). (M) Comparison of ChgA, Muc2, Gata4, and Fabp1 mRNA expression analysis by RT-qPCR from jejunal segments of the small intestine. Student's t-test was performed to determine p-values; expression was normalized to Gapdh. (Mean ± SEM: P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n = 3-6).