(A) Histological analysis for the comparison of cellular proliferation utilizing immunofluorescent staining for mitotic marker, phosphohistoneH3 (green) in wildtype and Klf5 loss-of-function mice (3, 5, 14, and 28 days post tamoxifen-induced recombination). Nuclear stain is DAPI (blue). (B) Quantification of average percentage of phosphohistoneH3 positive cells per crypt. Significance determined between groups of two (wildtype vs. loss of Klf5 for 3 days, wildtype vs. loss of Klf5 for 5 days, wildtype vs. loss of Klf5 for 14 days, wildtype vs. loss of Klf5 for 28 days, and loss of Klf5 for 3 vs. 28 days). (Mean ± SD, T-test: * P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n = 3)