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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Dig Dis Sci. 2014 Jul 29;60(1):86–100. doi: 10.1007/s10620-014-3307-z

Figure 5. Loss of Klf5 results in loss of active stem cell marker expression in the small intestine.

Figure 5

Results from of mRNA of expression for (A) cell cycle proteins ( CyclinD1, CyclinB1, and Cdk1) , (B) downstream targets of canonical Wnt signaling (Lgr5, Ascl2, Axin2, and CyclinD1), and (C) active intestinal stem cell markers (Lgr5, Ascl2, Axin2, CyclinD1) by RT-qPCR from jejunal segments of the small intestine. Student's t-test was performed to determine p-values; expression was normalized to Gapdh (SYBR) or Actb (Taqman). (Mean ± SEM: P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n = 3-6). Note that some qRT-PCR results for relative RNA expression appear on two graphs for comparison purposes.