. Author manuscript; available in PMC: 2016 Jan 1.

Published in final edited form as: Mol Ecol. 2014 Dec 31;24(1):151–179. doi: 10.1111/mec.13017

Table 5.

Gene ontology results for predicted genes that were differentially expressed (>1.5 fold difference, FDR P < 0.05) from the egg stage to day of eclosion in two populations of D. mojavensis reared on agria and organ pipe cacti. Life stages are defined in Table 3 and individual genes and their D. melanogaster ortholog names are listed when only several genes differed in expression and no functional clustering was observed in DAVID (Huang et al., 2009).

Population
	Las Bocas (mainland)				Punta Prieta (Baja California)
	OP>AG		AG>OP		OP>AG		AG>OP
	GOTerm (gene)	enrich score	GOTerm (gene)	enrich score	GOTerm (gene)	enrich score	GOTerm (gene)	enrich score
Egg	-	-	-	-	-	-	GI22080 - Cep78 protein	-
L1	1. cuticle, chitin structure	5.9^****	1. transport	5.9^****	-	-	GI24491 – Rheb	-
	1. cuticle, chitin structure		2. cell metabolism	4.3^****			GI24142 - Rab-protein 7 – GTPase mediated signal transduction
	2. Odorant binding, olfactory reception	2.1^***	3. protein transport	3.5^****
	2. Odorant binding, olfactory reception		4. Oxphos process/ ion transport	3.4^****
			5. mitotic spindle/ribosome protein	3.3^****			GI10537 - alternative testis transcripts open reading frame A
			6. cell morphogenesis	3.0^***
			7. RNA binding	3.0^**
			8. larval development	2.9^**			GI17029 - split ends - post-embryonic morphogenesis
			9. actin filament processes	2.9^**			GI17029 - split ends - post-embryonic morphogenesis
			10. protein synthesis	2.5^**
			11. RNA splicing	2.5^*
			12. axon development	2.2^**
			13.mitochondria transport peptide	2.1^**
			14. germ cell develop.	1.8^**
L2	1. cytoskeleton, ribosomal protein	1.1	1. metal ion binding	0.5	-	-	-	-
L3	-	-	GI16482 - ribosomal protein L40	-	-	-	-	-
			GI12755 – Rh50 (membrane transport)
			GI11050 – yu (ptn and RNA binding
EP	GI11311 - GNBP2 (Gram-negative bacteria binding protein 2)		GI18191 – fbp (fructose-1,6-bisphosphatase)	-	GI24313 - Cpr30F (pupal chitin structure)		1. ubl conjugation pathway, proteolysis	2.0 ^*
	GI11311 - GNBP2 (Gram-negative bacteria binding protein 2)		GI15376 - mesoderm development		GI19286
	GI24782⁺		GI15376 - mesoderm development
	GI12221⁺
LP	-	-	-	-	1. mitochondrial membrane	1.6	1. cuticle structure	1.2
0D	GI23541⁺	-	-	-	-	-	GI16633 – heat shock ptn/metal ion binding	-
							GI24251 – nucleotide binding
							GI16531 - Dpy-30 (adenylate kinase)
							GI19042

False Discovery rate P values

P < 0.05

^**

P < 0.01

^***

P < 0.001

^****

P < 0.0001

⁺

gene of unknown function