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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Clin Cancer Res. 2014 Oct 27;21(1):157–165. doi: 10.1158/1078-0432.CCR-14-0610

Figure 1.

Figure 1

CBL expression in human pancreatic ductal adenocarcinoma (PDAC) is inversely correlated with EGFR expression and activation. A, immunohistochemistry staining of human PDAC tumors stained for CBL, pEGFR, and EGFR reveals an inverse expression in 12 of 15 tumors (2 representative individual patient samples are shown), suggesting a possible regulatory effect of CBL on EGFR. B, correspondingly, when stimulated by EGF ligand, immunoblots of Panc-1 cells with CBL knockdown show increased phosphorylation of EGFR (Y1068) when compared to the isogenic parental cell line, with increased downstream pERK and pAKT (columns 2 versus 4). Relative densitometry values normalized to β-actin as a loading control are displayed above each phosphoprotein band and, C, plotted as a bar graph.